Research - Phyllostachys edulis / Bamboo
In Vitro Anti-Inflammatory and Wound-Healing Potential of a Phyllostachys edulis Leaf Extract – Identification of Isoorientin as an Active Compound
Jonas Wedler1, Tony Daubitz1, Götz Schlotterbeck2, Veronika Butterweck1
Extracts prepared from the leaves of Phyllostachys edulis (bamboo) have received attention in pharmacological research due to their potent antitumor, anti-inflammatory, antimicrobial, and anti-ulcerogenic activities. In this study, anti-inflammatory effects of a bamboo leaf extract on tumor necrosis factor alpha-induced overproduction of interleukin 8, vascular endothelial growth factor, and interleukin 6 in immortalized human keratinocytes were investigated for the first time. In addition, wound-healing effects were evaluated in 3T3-swiss albino mouse fibroblasts. Bamboo leaf extract and isoorientin inhibited the tumor necrosis factor alpha-induced release of interleukin 8 and vascular endothelial growth factor. Furthermore, isoorientin dose-dependently reduced levels of interleukin 6 in tumor necrosis factor alpha-α-treated immortalized human keratinocytes cells. Wound healing was evaluated using a modification of the classical scratch assay. For evaluation of the wound gap, a new computerized method based on time-lapse microscopy was developed. It was shown that bamboo leaf extract (10 µg/mL) improved wound closure by 28 % (12 h) and 54 % (24 h), respectively. In concentrations of 50 µg/mL and above, bamboo leaf extract inhibited cell migration without affecting cell viability. Isoorientin (10 µM) improved wound closure by 29 % (12 h) and 56 % (24 h), respectively. Comparable to bamboo leaf extract, higher concentrations of isoorientin prevented cell migration. It is suggested that bamboo leaf extract as well as isoorientin have a dual activity – in higher doses, they show anti-inflammatory effects, and in lower concentrations, they exert anti-angiogenic activities.
In conclusion, natural accelerators of cutaneous tissue repair with simultaneous anti-inflammatory activities are of great interest for a variety of dermatological disorders. Thus, treatment with P. edulis leaf extract or isoorientin may be a potential therapeutic strategy to promote wound healing and to prevent inflammation in a persistent inflammatory condition. Further investigations of the precise mechanism by which P. edulis and isoorientin reveal anti-inflammatory as well as wound-healing properties are currently underway.
Source : Planta Medica
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