Research - Quercetin
A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health
Yumei Lina1, Mary A. Murraya1, I. Ross Garretta2a3, Gloria E. Gutierreza2a4, Jeffry S. Nymana2a5, Gregory Mundya2a6 †, David Fasta7, Kevin W. Gellenbecka1 c1, Amitabh Chandraa7 and Shyam Ramakrishnana1a8
a1 Nutrilite Health Institute, 5600 Beach Boulevard, Buena Park, CA 90622, USA
a2 OsteoScreen Ltd, 2040 Babcock Road, San Antonio, TX 78023, USA
a3 9909 Charthouse Cove, Austin, TX 78730, USA
a4 Southwest Research Institute, 6220 Culebra Road, San Antonio, TX 78238, USA
a5 Department of Orthopaedic Surgery and Rehabilitation, Vanderbilt University Medical Center, Nashville, TN 37232, USA
a6 Vanderbilt University Medical Center, Nashville, TN 37232, USA
a7 Access Business Group, 7575 East Fulton Avenue, Ada, MI 49355, USA
a8 The Himalaya Drug Company, Makali, Tumkur Road, Bangalore – 562123, India
Using a sequential in vitro/in vivo approach, we tested the ability of botanical extracts to influence biomarkers associated with bone resorption and bone formation. Pomegranate fruit and grape seed extracts were found to exhibit anti-resorptive activity by inhibiting receptor activator of nuclear factor-κB ligand (RANKL) expression in MG-63 cells and to reduce IL-1β-stimulated calvarial 45Ca loss. A combination of pomegranate fruit and grape seed extracts were shown to be effective at inhibiting bone loss in ovariectomised rats as demonstrated by standard histomorphometry, biomechanical and bone mineral density measurements. Quercetin and licorice extract exhibited bone formation activity as measured by bone morphogenetic protein-2 (BMP-2) promoter activation, increased expression of BMP-2 mRNA and protein levels, and promotion of bone growth in cultured mouse calvariae. A combination of quercetin and licorice extract demonstrated a potential for increasing bone mineral density in an intact female rat model as compared with controls. The results from this sequential in vitro/in vivo research model yielded botanical extract formulas that demonstrate significant potential benefits for bone health.
Source : The Journal of Nutritional Sciences
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QUERCETIN ALLEVIATES BISPHENOL A-INDUCED CHANGES IN NUCLEIC ACID AND PROTEIN CONTENTS IN MICE
NEHA P. SANGAI and RAMTEJ J. VERMA*
Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad-380 009, Gujarat, India
The present study was an attempt to examine toxic effects of bisphenol A in liver and kidney of mice and its alleviation by quercetin. Oral administration of bisphenol A (60 and 120 mg/kg b. w./day) for 30 days caused, as compared to vehicle control significant, dose-dependent decrease in DNA, RNA and protein contents in liver and kidney of mice. Supplementation of quercetin (60 mg/kg b. w./day) along with bisphenol A
for 30 days caused, as compared to bisphenol A alone treated groups, significant alleviation in DNA, RNA and protein contents. The amelioration was comparatively higher for high dose bisphenol A plus quercetin treated group than that of low dose plus quercetin treated group.
....In conclusion, the present investigation has shown that bisphenol A is capable of producing alterations in biochemical parameters investigated in vital organs i.e., liver and kidney which are the organs responsible of detoxification of xenobiotics and foreign compounds. The alterations in biochemical parameters appeared to be more pronounced with the liver as compared to kidney. The 30 days treatment with bisphenol A revealed that bisphenol A might lead to DNA adduct formation with defect in RNA metabolism, which leads to impaired protein synthesis as DNA, RNA and protein contents decrease in case of bisphenol A treated animals Also, this study clarifies that the damage caused by plasticizer in mice can be recovered by combined treatment of quercetin and bisphenol A for 30 days. This may be attributed to the hepatoprotective, renoprotective
and antioxidative properties of quercetin.
Source : Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 68 No. 6 pp. 867ñ873, 2011
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