Research - Burns
Bee Pollen as a Promising Agent in the Burn Wounds Treatment
tPaweł Olczyk,1 Robert Koprowski,2 Justyna Kaźmierczak,3 Lukasz Mencner,3 Robert Wojtyczka,4 Jerzy Stojko,5 Krystyna Olczyk,3 and Katarzyna Komosinska-Vassev3
The aim of the present study was to visualize the benefits and advantages derived from preparations based on extracts of bee pollen as compared to pharmaceuticals commonly used in the treatment of burns. The bee pollen ointment was applied for the first time in topical burn treatment. Experimental burn wounds were inflicted on two white, domestic pigs. Clinical, histopathological, and microbiological assessment of specimens from burn wounds, inflicted on polish domestic pigs, treated with silver sulfadiazine or bee pollen ointment, was done. The comparative material was constituted by either tissues obtained from wounds treated with physiological saline or tissues obtained from wounds which were untreated. Clinical and histopathological evaluation showed that applied apitherapeutic agent reduces the healing time of burn wounds and positively affects the general condition of the animals. Moreover the used natural preparation proved to be highly effective antimicrobial agent, which was reflected in a reduction of the number of microorganisms in quantitative research and bactericidal activity of isolated strains. On the basis of the obtained bacteriological analysis, it may be concluded that the applied bee pollen ointment may affect the wound healing process of burn wounds, preventing infection of the newly formed tissue.
Source : Evidence Based Complementary and Alternative Medicine
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Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs
Fang Wu,1 Difei Bian,1 Yufeng Xia,1 Zhunan Gong,2 Qian Tan,3 Jiaojiao Chen,1 and Yue Dai1
1State Key Laboratory of Natural Medicines, Department of Pharmacology Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China
2Center for New Drug Research and Development, College of Life Science, Nanjing Normal University, Nanjing 210024, China
3Department of Burns and Plastic Surgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, China
Centella asiatica herbs have been prescribed as a traditional medicine for wound healing in China and Southeast Asia for a long time. They contain many kinds of triterpenoid compounds, mainly including glycosides (asiaticoside and madecassoside) and corresponding aglycones (asiatic acid and madecassic acid). To identify which is the major active constituent, a comprehensive and comparative study of these compounds was performed. In vitro, primary human skin fibroblasts, originating from healthy human foreskin samples, were treated with various concentrations of asiaticoside, madecassoside, asiatic acid, and madecassic acid, respectively. Cell proliferation, collagen synthesis, MMP-1/TIMP-1 balance, and TGF-β/Smad signaling pathway were investigated. In vivo, mice were orally administered with the four compounds mentioned above for two weeks after burn injury. The speed and quality of wound healing, as well as TGF-β1 levels in skin tissues, were examined. Interestingly, in contrast to prevalent postulations, asiaticoside and madecassoside themselves, rather than their corresponding metabolites asiatic acid and madecassic acid, are recognized as the main active constituents of C. asiatica herbs responsible for burn wound healing. Furthermore, madecassoside is more effective than asiaticoside (P=0.0446 for procollagen type III synthesis in vitro, P=0.0057 for wound healing speed, and 0.0491 for wound healing pattern in vivo, correspondingly).
Source : Evidence-Based Complementary and Alternative Medicine Volume 2012 (2012), Article ID 848093
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