Research - Black Alder / Alnus glutinosa(L.) Gaertn
Effect on Tumor Necrosis Factor-aProduction and Antioxidant Ability of Black Alder, as Factors Related to Its Anti-Inflammatory Properties
Nuria Acero1and Dolores Mun ̃oz-Mingarro2
Departments of1Biology and 2Chemistry, School of Pharmacy, CEU San Pablo University, Madrid, Spain.
ABSTRACT
Alders exhibit several uses in different areas and also offer some nutritional and medicinal values. The bark and leaves from black alder [Alnus glutinosa(L.) Gaertn] are used in folk medicine for the treatment of inflammatory processes and other health disorders. This study assessed if an extract ofA. glutinosastem bark exhibits some biological properties linked to improving the inflammatory state, which could partly justify its ethnopharmacological use. Therefore,various aspects of antioxidant activity as well as the effect on tumor necrosis factor-a(TNF-a) production were evaluated.The phytochemical study revealed the presence of terpenes, saponins, tannins, flavonoids, and anthraquinones (by high-performance thin-layer chromatography). The betulinic acid content in the extract, determined by reversed-phase high-performance liquid chromatography (validated method), was 0.72–0.027%. In addition, high amounts for total phenols as well as flavonoids were determined. The extract exhibited a 2,20-diphenylpicrylhydrazyl radical scavenging capacity similar to that of ascorbic acid and had a significant effect on superoxide anion scavenging, superior to that of ascorbic acid. It was alsoable to protect HeLa cells from induced oxidative stress. In the TNF-aassay, levels of this citokine were depressed by the extractin HL-60 cells. To test the effect of the extract on cell proliferation, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay was performed. According to the results, the antioxidant properties displayed by the extract o A. glutinosa stem bark, together with the effect on TNF-alevels, suggest that these activities, linked to a successful reduction in inflammatory processes, may support, in part, its ethnopharmacological use
Source : Journal of Medicinal Food
Link to Full Article
Nuria Acero1and Dolores Mun ̃oz-Mingarro2
Departments of1Biology and 2Chemistry, School of Pharmacy, CEU San Pablo University, Madrid, Spain.
ABSTRACT
Alders exhibit several uses in different areas and also offer some nutritional and medicinal values. The bark and leaves from black alder [Alnus glutinosa(L.) Gaertn] are used in folk medicine for the treatment of inflammatory processes and other health disorders. This study assessed if an extract ofA. glutinosastem bark exhibits some biological properties linked to improving the inflammatory state, which could partly justify its ethnopharmacological use. Therefore,various aspects of antioxidant activity as well as the effect on tumor necrosis factor-a(TNF-a) production were evaluated.The phytochemical study revealed the presence of terpenes, saponins, tannins, flavonoids, and anthraquinones (by high-performance thin-layer chromatography). The betulinic acid content in the extract, determined by reversed-phase high-performance liquid chromatography (validated method), was 0.72–0.027%. In addition, high amounts for total phenols as well as flavonoids were determined. The extract exhibited a 2,20-diphenylpicrylhydrazyl radical scavenging capacity similar to that of ascorbic acid and had a significant effect on superoxide anion scavenging, superior to that of ascorbic acid. It was alsoable to protect HeLa cells from induced oxidative stress. In the TNF-aassay, levels of this citokine were depressed by the extractin HL-60 cells. To test the effect of the extract on cell proliferation, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay was performed. According to the results, the antioxidant properties displayed by the extract o A. glutinosa stem bark, together with the effect on TNF-alevels, suggest that these activities, linked to a successful reduction in inflammatory processes, may support, in part, its ethnopharmacological use
Source : Journal of Medicinal Food
Link to Full Article